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In an effort to establish. Since our study shows that TOS4 is a. SBF-dependent, cell-cycle transcripts are induced in response. FM Bastos de Oliveira et al. For sbf or mbf Albuquerque To determine whether the transcriptional response to DNA replication stress can be generalized to all MBF-regulated genes or is limited to a small Albuqkerque of genes, we performed genome-wide expression analysis in cells responding to genotoxic agents For sbf or mbf Albuquerque induce DNA replication stress or DNA damage during S phase.
Identification of phosphorylated sites For the identification of the Nrm1 phosphopeptide containing phosphorylation on S, cells were treated with mM HU for 2 h and protein extract was prepared for phosphoproteomic analysis as previously described Albuquerque et al, Only postmitotic cells younger than three hours arrested cellular division in these conditions, while cells older than four hours were insensitive to the absence of growth factors.
The increase in Mbp1 binding to the TOS4 promo. Analysis was performed in G1 sync hronized cells released from a -factor.
So why use two non-redundant proteins to repress. bsf
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